Composite

Part:BBa_K092600

Designed by: EPF-Lausanne Team 2008   Group: iGEM08_EPF-Lausanne   (2008-10-19)


TetR cds with RBS and Terminator + pTetR, RFP with RBS

Composite part : combination of the part BBa_K092000 and the BBa_K092300 : The TetR sequence (with a ribosome binding site and terminator) is bound to the part containing the promoter of TetR, a ribosome binding site and the red fluorescent protein (RFP). Thus, the production of RFP directly depends on the production of TetR. The transcription of RFP is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1483
    Illegal AgeI site found at 1595
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None