Intermediate

Part:BBa_K091201

Designed by: Xiao Zhu   Group: iGEM08_Davidson-Missouri_Western   (2008-08-15)


plsrAcI overall testing construct

to test plsrA/cI hybrid promoter with GFP, supporting genes lsrK and lsrR, and lambda cI repressor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1158
    Illegal NheI site found at 4032
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1098
    Illegal BamHI site found at 1744
    Illegal BamHI site found at 3972
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1418
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 812


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Categories
Parameters
None