Device

Part:BBa_K091173

Designed by: Todd Eckdahl   Group: iGEM08_Davidson-Missouri_Western   (2008-08-04)


pLsr/cI+RBS+luxI+RBS+Mnt+TT+pTet/Mnt+RBS+luxI+RBS+cI+TT

This construct uses two hybrid promoters. pLsr/cI drives expression of the LuxI and Mnt genes. The promoter is designed to respond to repression by LsrR, which can be derepressed by phosphorylated AI-2, and to repression by lambda cI. pTet/Mnt drives expression of the LuxI and cI genes. It is designed for repression by the Tet repressor, which can be derepressed by aTc, and by the Mnt repressor. LuxI is a sythase that produces AI-1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 804
    Illegal BglII site found at 1333
    Illegal BglII site found at 2055
    Illegal XhoI site found at 1295
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None