Signalling

Part:BBa_K091164

Designed by: Andrew Gordon   Group: iGEM08_Davidson-Missouri_Western   (2008-07-28)


pLux/cI-RBS-LuxS-RBS-Mnt-TT

This construct is a modified promoter attched to sequences for an activator and repressor. The activator is LuxS, the AI-2 synthase. Both ribosome binding sites are the B0034 standard. The sequence is terminated by B0015, a double terminator. Mnt acts as the repressor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 238
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 336
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None