Signalling

Part:BBa_K091152

Designed by: Erin Feeney   Group: iGEM08_Davidson-Missouri_Western   (2008-07-18)


pLas' Testing Construct D

This part contains a constitutively active promoter, rbs, LuxR coding region, and LasR coding region followed by the pLas' promoter and GFP. K091151 generates both LasR and LuxR to test crosstalk and competitive binding with the pLas' promoter in the presence of AI-1 and PAI-1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1190
    Illegal AgeI site found at 1387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2591


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Parameters
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