Generator

Part:BBa_K091148

Designed by: Erin Feeney   Group: iGEM08_Davidson-Missouri_Western   (2008-07-17)


pLas' Promoter Testing Construct A

This part contains a constitutively active promoter, rbs, and LasR coding region followed by the pLas' promoter and GFP. K091148 generates the LasR necessary for for testing the pLas' and pLas+ Lux- promoters in the presence of PAI-1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1784


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