Generator

Part:BBa_K091130

Designed by: Pallavi Penumetcha and Kelly Davis   Group: iGEM08_Davidson-Missouri_Western   (2008-07-10)


pLacIQ controlled GFP generator

This device produces GFP under the control of the lacIQ promoter. The lacIQ promoter has a single base substitution in its -35 region and this increases its binding affinity for RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 727


[edit]
Categories
Parameters
None