Coding

Part:BBa_K091124

Designed by: Pallavi Penumetcha   Group: iGEM08_Davidson-Missouri_Western   (2008-07-09)

LacI_X86 protein

This is a variation of the LacI wild-type protein. It is characterized by a missense mutation in the 61st codon in which serine is mutated to leucine. This mutation causes an increase in binding affinity of this protein for the pLac promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None