Intermediate

Part:BBa_K091118

Designed by: James Barron   Group: iGEM08_Davidson-Missouri_Western   (2008-07-01)


codes for LasR and LasR regulated GFP

Has included LasR coding followed by transcriptional terminators followed by lasR promoter and GFP. Will fluoresce when ligated with an initial promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1002
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal AgeI site found at 537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1772


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Categories
Parameters
None