Regulatory

Part:BBa_K091104

Designed by: Robert Cool   Group: iGEM08_Davidson-Missouri_Western   (2008-06-19)

pLac/Mnt Hybrid Promoter

This promoter is a modified version of the Mnt promoter that is also responsive to LacI. The promoter should be repressed by Mnt repressor and also be repressed by LacI. In the absence of Mnt repressor and presence of LacI it should be induced by IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/negative
//regulation/multiple
Parameters
control
directionForward
negative_regulators2
o_h
o_l
positive_regulators