Regulatory
Part:BBa_K090501
Designed by: Daniel Goodman Group: iGEM08_Cambridge (2008-10-27)
Gram-Positive IPTG-Inducible Promoter
This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//rnap/prokaryote/subtilis/sigmaA
//direction/forward
//chassis/prokaryote/subtilis
//promoter
//regulation/negative
//direction/forward
//chassis/prokaryote/subtilis
//promoter
//regulation/negative
negative_regulators | 1 |
positive_regulators |