Deleted
Sample Discontinued
Experience: None
Not Used
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Device

Part:BBa_K084011

Designed by: Hiroki Fukutomi   Group: iGEM08_Chiba   (2008-09-18)

Time device

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

Time device

only AHL -> death

AHL + aTc (until the lux-lac hybrid promoter activate) -> express GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2118
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3330
    Illegal BsaI.rc site found at 2794
    Illegal BsaI.rc site found at 3013


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Parameters
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