Composite

Part:BBa_K081024

Designed by: Lorenzo Pasotti, Paolo Magni   Group: iGEM08_UNIPV-Pavia   (2008-10-19)


pcyA protein generator and Pomp

This device is a PoPS in -> pcyA out with Pomp promoter downstream.

Pomp (BBa_R0082) is a positively regulated, OmpR-controlled promoter. Phosphorylated OmpR binds to the three operator sites and activates transcription.

Proteins pcyA, ho1 and cph8 can produce a photosensitive complex that can activate the endogenous transcription factor OmpR. So, light (in particular 660 nm radiation) is an indirect repressor of Pomp.

These interactions are valid if used in E.coli deficient in natural EnvZ, because EnvZ can activate OmpR.

Pv K081024.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 790
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None