Regulatory

Part:BBa_K080000

Designed by: Jennifer Lei   Group: iGEM08_Bay_Area_RSI   (2008-10-20)


TCFbs-BMP4

Induction of BMP4 during early stages of cardiomyocyte differentiation results in inhibitory effects. From previous studies it is not clear whether BMP4 is necessary for cardiomyocyte differentiation. In our circuit, nkx2.5 and gata4 will be induced and initiate the pathways of cardiac differentiation. During normal cardiac muscle differentiation BMP4 is a downstream target of nkx-2.5 and gata4, the expression of which turns on at later stages of cardiac differentiation. To ensure that BMP4 has a delayed expression, we designed BMP4 under the control of minimal promoters of late markers of cardiac muscle differentiation, TCF and alpha cardiac actin.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 13
    Illegal SpeI site found at 946
    Illegal PstI site found at 293
    Illegal PstI site found at 993
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1565
    Illegal SpeI site found at 946
    Illegal PstI site found at 293
    Illegal PstI site found at 993
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 13
    Illegal SpeI site found at 946
    Illegal PstI site found at 293
    Illegal PstI site found at 993
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 13
    Illegal SpeI site found at 946
    Illegal PstI site found at 293
    Illegal PstI site found at 993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 419
    Illegal SapI.rc site found at 623


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