Reporter

Part:BBa_K079016

Designed by: Maria Vittoria Amaduzzi   Group: iGEM08_Bologna   (2008-10-24)


RecA promoter with GFP reporter protein on a medium copy number plasmid

The original BBa_J22106 part seemed to be not fully repressed when cloned into E. coli on a high copy number plasmid. Thus, we cloned the pRecA- GFP into the medium copy number plasmid pSB3K3 to study promoter regulation in this condition.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None