Regulatory

Part:BBa_K078101

Designed by: Gang Wu   Group: iGEM08_Beijing_Normal   (2008-10-22)


aromatic compounds regulatory pcbC promoter

This is a natural promoter from Pseudomonasp.DJ-12 activated by a series of aromatic compunds such as biphenyl, 4-chlorobiphenyl, 4-hydroxybiphenyl etl. It initiates transcrption of downstream genes pcbD and pcbC in DJ-12 when exposing to certain concentration of armotic pollutants. We implement this promoter as a sensor with a reporter gene fusion downstream to detect PCB related pollutant compounds.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None