Composite

Part:BBa_K077557

Designed by: Auke van Heel   Group: iGEM08_Groningen   (2008-10-11)

Part 1 of the Conways game of life system

This part constitutively produces LuxR, which forms a complex with HSL from neighboring cells. These LuxR.HSL complexes bind on the cI+LuxR promoter to produce LuxI, responsible for the HSL production to send signals to its own neighbors. Also GFP will be produced to visualize the ON state.

Input: 3OC6HSL

Output: LuxR, LuxI (producing 3OC6HSL) and GFP

K077557flow.jpg

Flow cytometry results of K077557. A total of 20.000 events were measured. Y-axis indicates number of events. X-axis indicates fluorescence on a logarithmic scale. Z-axis indicates time after induction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1766
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2442


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