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Part:BBa_K076005

Designed by: Sequence from publication (annotated)   Group: iGEM08_Princeton   (2008-10-29)

2A linker

This is a protein linker sequence that allows one protein, EYFP in the case of our plasmid pLV-TRE-Ngn1-linker-EYFP-2A-mKate-Ubc-Bla (BBa_K076000), to be linked to another, in our case mKate. The two proteins then are transcribed under the same promoter, but they act completely independently because the 2A amino acid sequence, once translated, is cleaved by the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None