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Part:BBa_K076003

Designed by: David Glass   Group: iGEM08_Princeton   (2008-10-29)

Linker

This short sequence can be used theoretically to connect any two proteins to form a fusion construct without disrupting the activity of either component. pLV-TRE-Ngn1-linker-EYFP-2A-mKate-Ubc-Bla (BBa_K076000) uses it to link Ngn1 (BBa_K076002) and EYFP (BBa_K076004).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None