Plasmid

Part:BBa_K076001

Designed by: Cil Purnick   Group: iGEM08_Princeton   (2008-10-29)

pLV-TRE-Sox17-Ubc-Bla

This is the backbone plasmid into which SfiI_Ngn1-linker-EYFP-2A-mKate_SfiI was inserted after digestion with SfiI. Cutting this backbone with SfiI cuts out the Sox17 gene and allows directional insertion of a properly designed insert.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal XbaI site found at 2875
    Illegal XbaI site found at 3298
    Illegal SpeI site found at 2802
    Illegal SpeI site found at 8621
    Illegal SpeI site found at 9295
    Illegal PstI site found at 3921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal NheI site found at 1572
    Illegal SpeI site found at 2802
    Illegal SpeI site found at 8621
    Illegal SpeI site found at 9295
    Illegal PstI site found at 3921
    Illegal NotI site found at 865
    Illegal NotI site found at 9331
    Illegal NotI site found at 10053
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal BglII site found at 626
    Illegal BglII site found at 4085
    Illegal BglII site found at 4151
    Illegal BglII site found at 4192
    Illegal BglII site found at 8384
    Illegal BglII site found at 9383
    Illegal BamHI site found at 1201
    Illegal XhoI site found at 2863
    Illegal XhoI site found at 9338
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal XbaI site found at 2875
    Illegal XbaI site found at 3298
    Illegal SpeI site found at 2802
    Illegal SpeI site found at 8621
    Illegal SpeI site found at 9295
    Illegal PstI site found at 3921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 328
    Illegal EcoRI site found at 1452
    Illegal EcoRI site found at 3304
    Illegal XbaI site found at 2875
    Illegal XbaI site found at 3298
    Illegal SpeI site found at 2802
    Illegal SpeI site found at 8621
    Illegal SpeI site found at 9295
    Illegal PstI site found at 3921
    Illegal NgoMIV site found at 843
    Illegal NgoMIV site found at 1095
    Illegal NgoMIV site found at 4780
    Illegal NgoMIV site found at 5813
    Illegal NgoMIV site found at 5874
    Illegal NgoMIV site found at 5988
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4174
    Illegal BsaI site found at 9365
    Illegal BsaI.rc site found at 2114
    Illegal BsaI.rc site found at 3934
    Illegal BsaI.rc site found at 7516
    Illegal BsaI.rc site found at 9254
    Illegal SapI site found at 6433
    Illegal SapI.rc site found at 10017


[edit]
Categories
Parameters
None