Inverter

Part:BBa_J85950

Designed by: Ron Weiss   Group: Weiss Lab   (2011-09-06)

LacI inverter regulated by TRE

TRE-tight-B1-LacI-T2A-EBFP2-FF5-miRFF4-scar2-Hef1a-LacO1Oid-B1-EYFP-FF4x4-scar3-Hef1a-B1-rtTa3-2A-NLS-mKate2

Composite part that forms a mammalian LacI inverter regulated by TRE

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2485
    Illegal EcoRI site found at 2681
    Illegal EcoRI site found at 3982
    Illegal EcoRI site found at 4923
    Illegal EcoRI site found at 6127
    Illegal XbaI site found at 30
    Illegal XbaI site found at 2640
    Illegal PstI site found at 3106
    Illegal PstI site found at 3611
    Illegal PstI site found at 4252
    Illegal PstI site found at 5254
    Illegal PstI site found at 5759
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2485
    Illegal EcoRI site found at 2681
    Illegal EcoRI site found at 3982
    Illegal EcoRI site found at 4923
    Illegal EcoRI site found at 6127
    Illegal NheI site found at 2982
    Illegal PstI site found at 3106
    Illegal PstI site found at 3611
    Illegal PstI site found at 4252
    Illegal PstI site found at 5254
    Illegal PstI site found at 5759
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2485
    Illegal EcoRI site found at 2681
    Illegal EcoRI site found at 3982
    Illegal EcoRI site found at 4923
    Illegal EcoRI site found at 6127
    Illegal BglII site found at 3360
    Illegal BglII site found at 5508
    Illegal BamHI site found at 2945
    Illegal BamHI site found at 4029
    Illegal BamHI site found at 6114
    Illegal XhoI site found at 2382
    Illegal XhoI site found at 3759
    Illegal XhoI site found at 5907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2485
    Illegal EcoRI site found at 2681
    Illegal EcoRI site found at 3982
    Illegal EcoRI site found at 4923
    Illegal EcoRI site found at 6127
    Illegal XbaI site found at 30
    Illegal XbaI site found at 2640
    Illegal PstI site found at 3106
    Illegal PstI site found at 3611
    Illegal PstI site found at 4252
    Illegal PstI site found at 5254
    Illegal PstI site found at 5759
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 2485
    Illegal EcoRI site found at 2681
    Illegal EcoRI site found at 3982
    Illegal EcoRI site found at 4923
    Illegal EcoRI site found at 6127
    Illegal XbaI site found at 30
    Illegal XbaI site found at 2640
    Illegal PstI site found at 3106
    Illegal PstI site found at 3611
    Illegal PstI site found at 4252
    Illegal PstI site found at 5254
    Illegal PstI site found at 5759
    Illegal NgoMIV site found at 3494
    Illegal NgoMIV site found at 5642
    Illegal AgeI site found at 2770
    Illegal AgeI site found at 4035
    Illegal AgeI site found at 5020
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7394
    Illegal BsaI.rc site found at 7583


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