Composite

Part:BBa_J85902

Designed by: Ron Weiss   Group: Weiss Lab   (2011-09-06)


Hef1a-LacO1Oid-B1-EYFP-FF4x4

Hef1a-LacO1Oid-B1-EYFP-FF4x4 Features:

  • Human elongation factor 1 alpha constitutive promoter
  • Lac operator 1
  • Lac operator ideal
  • (attB1 scar from recombination assembly)
  • Enhanced yellow FP
  • microRNA target site FF4 (four copies)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal PstI site found at 1577
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal NheI site found at 307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal PstI site found at 1577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal BglII site found at 685
    Illegal BamHI site found at 270
    Illegal BamHI site found at 1354
    Illegal XhoI site found at 1084
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal PstI site found at 1577
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1307
    Illegal PstI site found at 431
    Illegal PstI site found at 936
    Illegal PstI site found at 1577
    Illegal NgoMIV site found at 819
    Illegal AgeI site found at 95
    Illegal AgeI site found at 1360
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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