Composite
Part:BBa_J85902
Designed by: Ron Weiss Group: Weiss Lab (2011-09-06)
Hef1a-LacO1Oid-B1-EYFP-FF4x4
Hef1a-LacO1Oid-B1-EYFP-FF4x4 Features:
- Human elongation factor 1 alpha constitutive promoter
- Lac operator 1
- Lac operator ideal
- (attB1 scar from recombination assembly)
- Enhanced yellow FP
- microRNA target site FF4 (four copies)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1307
Illegal PstI site found at 431
Illegal PstI site found at 936
Illegal PstI site found at 1577 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1307
Illegal NheI site found at 307
Illegal PstI site found at 431
Illegal PstI site found at 936
Illegal PstI site found at 1577 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1307
Illegal BglII site found at 685
Illegal BamHI site found at 270
Illegal BamHI site found at 1354
Illegal XhoI site found at 1084 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1307
Illegal PstI site found at 431
Illegal PstI site found at 936
Illegal PstI site found at 1577 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 1307
Illegal PstI site found at 431
Illegal PstI site found at 936
Illegal PstI site found at 1577
Illegal NgoMIV site found at 819
Illegal AgeI site found at 95
Illegal AgeI site found at 1360 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |