Composite

Part:BBa_J85901

Designed by: Ron Weiss   Group: Weiss Lab   (2011-09-06)


Hef1a-B1-rtTA3-2A-NLS-mKate2

Hef1a-B1-rtTA3-2A-NLS-mKate2

Constitutive promoter Hef1a regulates production of rtTA3 transcriptional activator and nuclear-localized FP mKate

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal BglII site found at 591
    Illegal BamHI site found at 1197
    Illegal XhoI site found at 990
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal NgoMIV site found at 725
    Illegal AgeI site found at 103
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2477
    Illegal BsaI.rc site found at 2666


[edit]
Categories
Parameters
None