Coding

Part:BBa_J85800

Designed by: Ron Weiss   Group: Weiss Lab   (2011-09-06)


LacI-T2A-EBFP2-FF5-miRFF4

LacI-T2A-EBFP2-FF5-miRFF4 Features:

  • transcriptional repressor LacI
  • 2A translational fusion tag
  • enhanced blue fluorescent protein EBFP2
  • microRNA target site FF5
  • microRNA FF4

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2133
    Illegal XhoI site found at 2030
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2133
  • 1000
    COMPATIBLE WITH RFC[1000]


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