Inverter

Part:BBa_J85209

Designed by: Jonathan W Babb   Group: Weiss Lab   (2010-01-28)

J85204.J85101

Composite part to detect 3OC6HSL and produce RFP, or in the absence of 30C6HSL to produce GFP and cm resistance. Uses TetR/Ptet regulatory mechanism. Same as J85204 but with Chloramphenicol resistance added. J58208 is a sister part with tetracycline resistance instead.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 1737
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987
    Illegal BsaI.rc site found at 3356


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Categories
Parameters
None