Inverter

Part:BBa_J85208

Designed by: Jonathan W Babb   Group: Weiss Lab   (2010-01-28)

J85204.J85206

Composite part to detect 3OC6HSL and produce RFP, or in the absence of 30C6HSL to produce GFP and tetracycline resistance. Uses TetR/Ptet regulatory mechanism. Based on J85204. Expect slow growth when tetracycline resistance is not repressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3602
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3748
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3774
    Illegal NgoMIV site found at 4142
    Illegal NgoMIV site found at 4302
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 1737
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987
    Illegal BsaI.rc site found at 3356


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Categories
Parameters
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