Device

Part:BBa_J75005

Designed by: Mr Kyle C Grant   Group: Jaramillo Lab   (2015-01-07)


A Complete Repressilator Device

The DNA sequence is based on the plasmid map from the Elowitz paper (http://www.elowitz.caltech.edu/publications/Repressilator.pdf). Sequences were derived from current BioBricks where labelled and other paper sources.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3960
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 101
    Illegal NheI site found at 124
    Illegal SpeI site found at 3961
    Illegal PstI site found at 3975
    Illegal NotI site found at 7
    Illegal NotI site found at 3968
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 3961
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 3961
    Illegal PstI site found at 3975
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None