Plasmid_Backbone

Part:BBa_J73999

Designed by: Junling Huo   Group: Miller Lab   (2009-12-10)


plasmid vector for Rhodobacter sphaeroides

This vector is based on pMG170/pMG171. LacZ promoter and lac reporter have been removed from original vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 2468
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 4636
    Illegal XhoI site found at 28
    Illegal XhoI site found at 3463
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 4620
    Illegal NgoMIV site found at 4644
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 513


[edit]
Categories
Parameters
None