Cell

Part:BBa_J72214

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)


MC1061 upp::pir+FRT-trimethoprimR-FRT

MC1061 modified to express pir from plasmid R6K. The sequence is for variant E of an RBS library on pir that resulted in several published variants A-J (PMID 21787416). Strain also known as JTK164.

Composition of the insertion: BBa_J72177.rbslib-BBa_J72162.BBa_J72049.BBa_J27001.BBa_J72073.BBa_J72165.BBa_J72166.BBa_J27001

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2435
    Illegal XbaI site found at 2826
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1309
    Illegal NheI site found at 1332
    Illegal NheI site found at 2466
    Illegal NheI site found at 2489
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3655
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2435
    Illegal XbaI site found at 2826
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2435
    Illegal XbaI site found at 2826
    Illegal AgeI site found at 3121
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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