Cell

Part:BBa_J72213

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

MC1061 O16::repA+FRT-kanR-FRT

MC1061 modified to express repA from the ColE2-P9 plasmid. The sequence is for variant I of an RBS library on repA that resulted in several published variants A-J (PMID 21787416). Strain also known as JTK160.
Composition of insertion: BBa_J72073.rbslib-BBa_J72160.BBa_J72049.BBa_J27001.BBa_J72092.BBa_J27001

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1628
    Illegal XbaI site found at 2787
    Illegal XbaI site found at 3856
    Illegal PstI site found at 137
    Illegal PstI site found at 2758
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1628
    Illegal NheI site found at 1649
    Illegal NheI site found at 1672
    Illegal PstI site found at 137
    Illegal PstI site found at 2758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2743
    Illegal EcoRI site found at 1628
    Illegal BglII site found at 1637
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1628
    Illegal XbaI site found at 2787
    Illegal XbaI site found at 3856
    Illegal PstI site found at 137
    Illegal PstI site found at 2758
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1628
    Illegal XbaI site found at 2787
    Illegal XbaI site found at 3856
    Illegal PstI site found at 137
    Illegal PstI site found at 2758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


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