Plasmid_Backbone

Part:BBa_J72211

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

ampicillin resistant p15A vector with dapD

ampicillin resistant p15A vector with dapD

referred to internally as pBjk3066A

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3259
    Illegal PstI site found at 1687
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3259
    Illegal NheI site found at 1015
    Illegal PstI site found at 1687
    Illegal NotI site found at 1050
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a suffix.
    Illegal BamHI site found at 1
    Illegal XhoI site found at 2063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3259
    Illegal PstI site found at 1687
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3259
    Illegal PstI site found at 1687
    Illegal AgeI site found at 605
    Illegal AgeI site found at 929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2920


[edit]
Categories
Parameters
None