Plasmid_Backbone

Part:BBa_J72210

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site

Kanamycin resistant BAC with R6K origin, glucose driven promoter 5' of cloning site. Can be maintained at higher copy in strains expressing pir.

Referred to internally as pBacRJ2g

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6673
    Illegal XbaI site found at 4369
    Illegal SpeI site found at 839
    Illegal PstI site found at 1999
    Illegal PstI site found at 3540
    Illegal PstI site found at 5062
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6673
    Illegal NheI site found at 6657
    Illegal SpeI site found at 839
    Illegal PstI site found at 1999
    Illegal PstI site found at 3540
    Illegal PstI site found at 5062
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a suffix.
    Illegal BglII site found at 2348
    Illegal BglII site found at 4415
    Illegal BglII site found at 4849
    Illegal BamHI site found at 1
    Illegal XhoI site found at 4548
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6673
    Illegal XbaI site found at 4369
    Illegal SpeI site found at 839
    Illegal PstI site found at 1999
    Illegal PstI site found at 3540
    Illegal PstI site found at 5062
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 6673
    Illegal XbaI site found at 4369
    Illegal SpeI site found at 839
    Illegal PstI site found at 1999
    Illegal PstI site found at 3540
    Illegal PstI site found at 5062
    Illegal NgoMIV site found at 3858
    Illegal NgoMIV site found at 5513
    Illegal AgeI site found at 1611
    Illegal AgeI site found at 2504
    Illegal AgeI site found at 3734
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 757
    Illegal BsaI.rc site found at 5684
    Illegal BsaI.rc site found at 6250
    Illegal BsaI.rc site found at 6428
    Illegal SapI.rc site found at 1754
    Illegal SapI.rc site found at 2964
    Illegal SapI.rc site found at 5362
    Illegal SapI.rc site found at 5572


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