Plasmid_Backbone

Part:BBa_J72202

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

ampicillin resistant p15A vector

ampicillin resistant p15A vector

referred to internally as pBjo1601A

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2261
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2261
    Illegal NheI site found at 1015
    Illegal NotI site found at 1050
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a suffix.
    Illegal BamHI site found at 1
    Illegal XhoI site found at 1065
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2261
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2261
    Illegal AgeI site found at 605
    Illegal AgeI site found at 929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1922


[edit]
Categories
Parameters
None