Composite

Part:BBa_J72198

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

Payload Delivery Device with Barnase Payload

Detects endocytosis, causes self lysis, and causes endosome lysis, releasing cell contents into the cytoplasm. Delivers Barnase to recipient cells.

Composition: BBa_J72054.BBa_J72052.BBa_K112305.BBa_K112311.BBa_J72049.BBa_J72050.BBa_K112317.BBa_J72051.BBa_J72005.BBa_J72066. BBa_J72129.BBa_J72068.BBa_J72128.BBa_J72059.BBa_J72074.BBa_J72178.BBa_J72065.BBa_J72173.BBa_J72077.BBa_J72174.BBa_J72175.BBa_J72049

Referred to internally as jtk3060
Sequence available upon request only.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None