Coding

Part:BBa_J72170

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

K1 biosynthesis cluster

Biosynthetic cluster responsible for expression of the K1 capsule, from ATCC 23503. Does not appear to be functional. Referred to internally as lts6020.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 12452
    Illegal PstI site found at 1150
    Illegal PstI site found at 5080
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1640
    Illegal PstI site found at 1150
    Illegal PstI site found at 5080
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 12452
    Illegal PstI site found at 1150
    Illegal PstI site found at 5080
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 12452
    Illegal PstI site found at 1150
    Illegal PstI site found at 5080
    Illegal NgoMIV site found at 4090
    Illegal AgeI site found at 3217
    Illegal AgeI site found at 5455
    Illegal AgeI site found at 15855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13523
    Illegal SapI site found at 10073


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Categories
Parameters
None