Coding

Part:BBa_J72165

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

rbs.DHFR, TrimR

Dihydrofolate reductase (Type II, from plasmid R-388) with strong synthetic RBS. Gene expression confers resistance to trimethoprim. Referred to internally as jtk3021.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None