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Part:BBa_J72161

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-09-17)

ColE2 origin of replication

Origin of replication from the ColE2-P9 plasmid, including the minimal origin and surrounding sequence. Provides for stable plasmid replication in the presence of RepA (akin to the pir/R6K system). Referred to internally as jtk2642.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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