Plasmid_Backbone

Part:BBa_J72154

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-08-12)


kanamycin + chloramphenicol resistant p15A vector (2ab vector)

Referred to internally as pBjh1601KC

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3132
    Illegal PstI site found at 1959
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3132
    Illegal NheI site found at 1015
    Illegal PstI site found at 1959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a suffix.
    Illegal BamHI site found at 1
    Illegal XhoI site found at 1999
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3132
    Illegal PstI site found at 1959
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3132
    Illegal PstI site found at 1959
    Illegal AgeI site found at 605
    Illegal AgeI site found at 929
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None