Composite

Part:BBa_J72152

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-08-12)

Arabinose Inducible Phagemid with GFP

{araC-Pbad}{rbs.coi}{double terminator}{cin, no start, frameshift}{repL no start}{LPsit}{rbs.pacA}{TrnpB}{Ptet}{rbs.sfGFP}{double terminator}

Composition: bba_J72071.bba_J72082.bba_J72049.bba_J72079.bba_J72080.bba_J72076.bba_J72083.bba_J72077.bba_J72005.bba_J72048.bba_J72049

Part is referred to internally as jtk3410.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1023
    Illegal AgeI site found at 2469
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005
    Illegal SapI.rc site found at 1840
    Illegal SapI.rc site found at 4831


[edit]
Categories
Parameters
None