Plasmid_Backbone

Part:BBa_J72112

Designed by: Josh Kittleson   Group: Anderson Lab   (2011-11-09)

SpecR split R6K vector

Requires pir gene provided in trans to replicate. Part is referred to internally as pBjk2807.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2109
    Illegal XbaI site found at 334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a suffix.
    Illegal BamHI site found at 1
    Illegal XhoI site found at 1487
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2109
    Illegal XbaI site found at 334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2109
    Illegal XbaI site found at 334
    Illegal NgoMIV site found at 1340
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None