Part:BBa_J72104

{rhaRS_PrhaB}{luxCDABE}{vioABCDE}{double terminator}{Pcon-C5}{rbs.lacZ}

Large reporter construct. Composition: bba_J72072.bba_J72091.bba_J72090.bba_J72049.bba_J72074.bba_J72087
Part is referred to internally as jtk3015.

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This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 4047
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 295
  Illegal NheI site found at 8928
  Illegal NheI site found at 15392
  Illegal NheI site found at 15415
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 4047
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 4047
  Illegal NgoMIV site found at 11713
  Illegal AgeI site found at 1592
  Illegal AgeI site found at 5025
  Illegal AgeI site found at 11909
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3406
  Illegal SapI site found at 5544
  Illegal SapI.rc site found at 14451
  Illegal SapI.rc site found at 14526