Composite

Part:BBa_J72097

Designed by: Josh Kittleson   Group: Anderson Lab   (2011-11-09)

{rhaRS_PrhaB}{rbs.coi}{double terminator}

Rhamnose inducible coi expression cassette. Will induce lysis of a P1 lysogen. Composition: bba_J72072.bba_J72082.bba_J72049
Part is referred to internally as jtk2655.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 295
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1592
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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