Coding

Part:BBa_J72082

Designed by: Josh Kittleson   Group: Anderson Lab   (2011-11-09)

{rbs.coi} in BglBrick Format

Coi is an anti-repressor that interacts with and stoichiometrically disables the master repressor of bacteriphage P1, c1. For details of wild type behavior, see PMID 15489417. Heterologous expression of sufficient coi in a P1 lysogenized E. coli strain results in induction of the lytic cycle. Part is referred to internally as bca1265.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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