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Part:BBa_J72081

Designed by: Josh Kittleson   Group: Anderson Lab   (2011-11-09)

{p15a origin of replication} in BglBrick Format

The p15a origin of replication, which maintains plasmids in E. coli at moderate copy number (15-30ish). Part is referred to internally as jtk3017.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 917
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 507
    Illegal AgeI site found at 831
  • 1000
    COMPATIBLE WITH RFC[1000]


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