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Part:BBa_J72080

Designed by: Josh Kittleson   Group: Anderson Lab   (2011-11-09)

{repL, no start} in BglBrick Format

The protein RepL initiates lytic replication of bacteriphage P1 from an origin within the coding region of the repL gene. This version of repL is intended to be noncoding, and only present the cis element required to act as a lytic origin. For a description of wildtype function, see PMID 15489417. Part is referred to internally as jtk2542.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 272
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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