Regulatory

Part:BBa_J72071

Designed by: Josh Kittleson   Group: Anderson Lab   (2011-11-08)

{araC-Pbad} in BglBrick Format

The arabinose binding protein AraC is divergently transcribed from the promoter it regulates. Shows a approximately ~500-1000 fold increase in expression upon induction with arabinose. Full induction observed at 0.2% (w/v) arabinose. Part is referred to internally as bca1109.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1005


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Categories
Parameters
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