Plasmid

Part:BBa_J72043

Designed by: Jennifer Brophy   Group: Anderson Lab   (2010-07-15)


pMLLKA 2AB Construction Plasmid

2AB Assembly Plasmid with Kanamycin and Ampicillin resistance.


This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2868
    Illegal PstI site found at 1276
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2868
    Illegal PstI site found at 1276
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2868
    Illegal XhoI site found at 1270
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2868
    Illegal PstI site found at 1276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2868
    Illegal PstI site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1015


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Categories
Parameters
None