Composite

Part:BBa_J72015

Designed by: John Anderson   Group: Anderson Lab   (2008-07-25)

Genomically Integrated BamHI expression device in BBb

This sequence is the plasmid that would result from flp-mediated excision applied to part J72007(in house, pBca9364). Strain MC1061::J72015 is the physical manifestation of integrating J72007 into the MC1061 genome by phi80 att site integration, and excision with flp recombinase.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 23
    Illegal SpeI site found at 211
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 458
    Illegal SpeI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 23
    Illegal SpeI site found at 211
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 23
    Illegal SpeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


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