Composite

Part:BBa_J72014

Designed by: John Anderson   Group: Anderson Lab   (2008-07-25)

Genomically Integrated BglII expression device in BBb

This sequence is the plasmid that would result from flp-mediated excision applied to part J72013 (in house, pBca9365). Strain MC1061::J72014 is the physical manifestation of product of integration of J72013 into the MC1061 genome by phi80 att site integration, and excision with flp-recombinase.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1060
    Illegal XbaI site found at 1192
    Illegal SpeI site found at 1380
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1627
    Illegal SpeI site found at 1380
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1060
    Illegal XbaI site found at 1192
    Illegal SpeI site found at 1380
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1060
    Illegal XbaI site found at 1192
    Illegal SpeI site found at 1380
    Illegal NgoMIV site found at 540
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 669
    Illegal SapI.rc site found at 1082


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