Coding

Part:BBa_J72012

Designed by: John Anderson   Group: Anderson Lab   (2008-07-25)


{M.BglII} in BBb

BglII methyltransferase open reading frame basic part. Methylates the sequence AGATCT.

This part is in BBb Format. It is flanked by BglII and BamHI sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 975
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 975
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 975
    Illegal NgoMIV site found at 455
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 584
    Illegal SapI.rc site found at 997


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Parameters
None